rosettesep human monocytes enrichment cocktail Search Results


rosettesep monocyte enrichment kit  (STEMCELL Technologies Inc)
90
STEMCELL Technologies Inc rosettesep monocyte enrichment kit
Rosettesep Monocyte Enrichment Kit, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rosettesep monocyte enrichment kit/product/STEMCELL Technologies Inc
Average 90 stars, based on 1 article reviews
rosettesep monocyte enrichment kit - by Bioz Stars, 2026-03
90/100 stars
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rosettesep human monocyte enrichment cocktail 15068  (CellSystems Biotechnologie Vertrieb GmbH)
90
CellSystems Biotechnologie Vertrieb GmbH rosettesep human monocyte enrichment cocktail 15068
A) MDM were generated from CD14++ monocytes purified from PBMC by MACS separation followed by 5-day incubation with M-CSF (100 ng/ml) . Cells remained untreated (none) or were stimulated with LPS (10 ng/ml) or with particle-mix of ultrafine P90 and fine TiO 2 (each with 32 μg/ml) for 3 h (n = 5 patients with COPD, n = 8 healthy controls, mean ± S.D.; * p < 0.05 compared to untreated controls). B) Effect of particles on CYP1B1 expression in sputum macrophages of healthy non-smokers, healthy smokers and patients with COPD. Sputum macrophages were purified using <t>RosetteSep</t> to deplete unwanted leukocytes. Cells remained untreated (none) or were stimulated with LPS (10 ng/ml) or with particle-mix of ultrafine P90 and fine TiO 2 (each with 32 μg/ml) for 3 h (n = 5 non-smokers, n = 4 smokers, n = 7 patients with COPD, mean ± S.D.; * p < 0.05). C) Cells remained untreated (none) or were stimulated with LPS (10 ng/ml), with particle mix of ultrafine P90 and fine TiO 2 (each with 32 μg/ml) or with each particle separately for 22 h (each with 32 μg/ml) (A549 n = 3, Calu-3 n = 4 experiments from different cell passages, mean ± S.D.; * p < 0.05 compared to untreated control). D) Effect of ultrafine P90 on CYP1B1 mRNA expressin in primary bronchial epithelial cells. Cells were obtained by bronchial brush biopsy. Cells remained untreated (none) or were stimulated with ultrafine P90 (32 μg/ml) for 3 h (n = 7 incubations from different donors, mean ± S.D.; * p < 0.05 compared to untreated control).
Rosettesep Human Monocyte Enrichment Cocktail 15068, supplied by CellSystems Biotechnologie Vertrieb GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rosettesep human monocyte enrichment cocktail 15068/product/CellSystems Biotechnologie Vertrieb GmbH
Average 90 stars, based on 1 article reviews
rosettesep human monocyte enrichment cocktail 15068 - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier
immunodensity negative selection rosettesep human monocyte enrichment cocktail  (STEMCELL Technologies Inc)
90
STEMCELL Technologies Inc immunodensity negative selection rosettesep human monocyte enrichment cocktail
A) MDM were generated from CD14++ monocytes purified from PBMC by MACS separation followed by 5-day incubation with M-CSF (100 ng/ml) . Cells remained untreated (none) or were stimulated with LPS (10 ng/ml) or with particle-mix of ultrafine P90 and fine TiO 2 (each with 32 μg/ml) for 3 h (n = 5 patients with COPD, n = 8 healthy controls, mean ± S.D.; * p < 0.05 compared to untreated controls). B) Effect of particles on CYP1B1 expression in sputum macrophages of healthy non-smokers, healthy smokers and patients with COPD. Sputum macrophages were purified using <t>RosetteSep</t> to deplete unwanted leukocytes. Cells remained untreated (none) or were stimulated with LPS (10 ng/ml) or with particle-mix of ultrafine P90 and fine TiO 2 (each with 32 μg/ml) for 3 h (n = 5 non-smokers, n = 4 smokers, n = 7 patients with COPD, mean ± S.D.; * p < 0.05). C) Cells remained untreated (none) or were stimulated with LPS (10 ng/ml), with particle mix of ultrafine P90 and fine TiO 2 (each with 32 μg/ml) or with each particle separately for 22 h (each with 32 μg/ml) (A549 n = 3, Calu-3 n = 4 experiments from different cell passages, mean ± S.D.; * p < 0.05 compared to untreated control). D) Effect of ultrafine P90 on CYP1B1 mRNA expressin in primary bronchial epithelial cells. Cells were obtained by bronchial brush biopsy. Cells remained untreated (none) or were stimulated with ultrafine P90 (32 μg/ml) for 3 h (n = 7 incubations from different donors, mean ± S.D.; * p < 0.05 compared to untreated control).
Immunodensity Negative Selection Rosettesep Human Monocyte Enrichment Cocktail, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/immunodensity negative selection rosettesep human monocyte enrichment cocktail/product/STEMCELL Technologies Inc
Average 90 stars, based on 1 article reviews
immunodensity negative selection rosettesep human monocyte enrichment cocktail - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier

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A) MDM were generated from CD14++ monocytes purified from PBMC by MACS separation followed by 5-day incubation with M-CSF (100 ng/ml) . Cells remained untreated (none) or were stimulated with LPS (10 ng/ml) or with particle-mix of ultrafine P90 and fine TiO 2 (each with 32 μg/ml) for 3 h (n = 5 patients with COPD, n = 8 healthy controls, mean ± S.D.; * p < 0.05 compared to untreated controls). B) Effect of particles on CYP1B1 expression in sputum macrophages of healthy non-smokers, healthy smokers and patients with COPD. Sputum macrophages were purified using RosetteSep to deplete unwanted leukocytes. Cells remained untreated (none) or were stimulated with LPS (10 ng/ml) or with particle-mix of ultrafine P90 and fine TiO 2 (each with 32 μg/ml) for 3 h (n = 5 non-smokers, n = 4 smokers, n = 7 patients with COPD, mean ± S.D.; * p < 0.05). C) Cells remained untreated (none) or were stimulated with LPS (10 ng/ml), with particle mix of ultrafine P90 and fine TiO 2 (each with 32 μg/ml) or with each particle separately for 22 h (each with 32 μg/ml) (A549 n = 3, Calu-3 n = 4 experiments from different cell passages, mean ± S.D.; * p < 0.05 compared to untreated control). D) Effect of ultrafine P90 on CYP1B1 mRNA expressin in primary bronchial epithelial cells. Cells were obtained by bronchial brush biopsy. Cells remained untreated (none) or were stimulated with ultrafine P90 (32 μg/ml) for 3 h (n = 7 incubations from different donors, mean ± S.D.; * p < 0.05 compared to untreated control).

Journal: Particle and Fibre Toxicology

Article Title: Ultrafine carbon particles down-regulate CYP1B1 expression in human monocytes

doi: 10.1186/1743-8977-6-27

Figure Lengend Snippet: A) MDM were generated from CD14++ monocytes purified from PBMC by MACS separation followed by 5-day incubation with M-CSF (100 ng/ml) . Cells remained untreated (none) or were stimulated with LPS (10 ng/ml) or with particle-mix of ultrafine P90 and fine TiO 2 (each with 32 μg/ml) for 3 h (n = 5 patients with COPD, n = 8 healthy controls, mean ± S.D.; * p < 0.05 compared to untreated controls). B) Effect of particles on CYP1B1 expression in sputum macrophages of healthy non-smokers, healthy smokers and patients with COPD. Sputum macrophages were purified using RosetteSep to deplete unwanted leukocytes. Cells remained untreated (none) or were stimulated with LPS (10 ng/ml) or with particle-mix of ultrafine P90 and fine TiO 2 (each with 32 μg/ml) for 3 h (n = 5 non-smokers, n = 4 smokers, n = 7 patients with COPD, mean ± S.D.; * p < 0.05). C) Cells remained untreated (none) or were stimulated with LPS (10 ng/ml), with particle mix of ultrafine P90 and fine TiO 2 (each with 32 μg/ml) or with each particle separately for 22 h (each with 32 μg/ml) (A549 n = 3, Calu-3 n = 4 experiments from different cell passages, mean ± S.D.; * p < 0.05 compared to untreated control). D) Effect of ultrafine P90 on CYP1B1 mRNA expressin in primary bronchial epithelial cells. Cells were obtained by bronchial brush biopsy. Cells remained untreated (none) or were stimulated with ultrafine P90 (32 μg/ml) for 3 h (n = 7 incubations from different donors, mean ± S.D.; * p < 0.05 compared to untreated control).

Article Snippet: 10 μl packed erythrocytes and 50 μl RosetteSep Human Monocyte Enrichment Cocktail (15068, CellSystems, St. Katharinen, Germany) were added, which crosslinks unwanted cells to multiple red blood cells, forming immunorosettes.

Techniques: Generated, Purification, Incubation, Expressing, Control